Carcinogenesis, Teratogenesis & Mutagenesis ›› 2005, Vol. 17 ›› Issue (4): 198-201.doi: 10.3969/j.issn.1004-616x.2005.04.002

Previous Articles     Next Articles

The Cell Strains Derived from Different Morphologic Colonies in the Process of Malignant Trans- formation of Human Bronchial Epithelial Cells by Thiotepa

ZHANG Chuang-ye;YUAN Su-bo;WU Chun-qi;QU Wen-sheng   

  1. National Beijing Center for Drug Safety Evaluation and Research，Institute of Pharmacology and Toxicology，Academy of Military Medical Sciences，Beijing 100850，China
  • Received:2004-12-14 Revised:2005-01-19 Online:2005-07-30 Published:2005-07-30
  • Contact: YUAN Su-bo

PDF (PC)

3081

Abstract: BACKGROUND ＆ AIM： To study the changes of cellulosity and tumorigenicity of the two cell strains derived from different morphologic colonies in the process of malignant transformation of human bronchial epithelial cell strain 2B(BEAS-2B) treated with thiotepa. MATERIAL AND METHODS: The two different morphologic colonies, which derived from transformed BEAS-2B cells by thiotepa, were isolated and cultivated respectively. We established the two cell strains and named them human bronchial epithelial cell strain S(BEAS-S) and human bronchial epithelial cell strain C(BEAS-C).With the passage, the immunocytochemistry stain,cellular agglutination test and anchorage independence test were used to analyze their changes of cellulosity and tumorigenicity. RESULTS:With the passage, the basal cell's special marker cytokeration 34βE12 express increased in BEAS-C cells as its cellular agglutination and tumorigenicity,but these don't change obviously in the BEAS-2B cells and BEAS-S cells.CONCLUSION: The basal cell might be the stem cell of malignant transformation of human bronchial epithelial cells. Meanwhile, the morphometric difference of these cells and colonies provided valuable information to the morphometrig research of transformation of human-originated cells.

Key words: thiotepa, immortalized human bronchial epithelial cells, malignant transformation, morphometry, immunocytochemistry stain